Institution: Institute for Insectbiotechnology, Fraunhofer IME/JLU Gießen

TBG group members

  • Andreas Vilcinskas
  • Miray Tonk
  • Bjoern M von Reumont

Other members (Fraunhofer IME & JLU):

  • Lea Thalmann
  • Anne Pöppel
  • Frank Förster
  • Andre Billion
  • Sarah Lenke (JLU)
  • Kim Kirchhoff (PhD)
  • Tim Lüddeke (PhD)
  • Rabia Oezbek (PhD)
  • Stephan Druekwitz (PhD)

Group Animal Venomics

TBG project summary

Venomics represents an innovative approach combining sensitive –omics techniques to identify transcripts encoding venom toxins. The tremendous diversity of venomous animals provides an indispensable biologic resource that is now being investigated in the project “Animal Venomics” as a source for valuable new compounds. Taking advantage of bioinformatic tools for sequence-based drug discovery we sequence the genomes of venomous animals and the transcriptomes of their venomous glands in order to identify novel peptides or proteins with therapeutic potential. Novel candidate peptides or proteins are produced as synthetic or recombinant analogues to facilitate their biological profiling and the screening of their potential applications in medicine and plant protection. Finally, we use the combined proteome and transcriptome data to annotate the genomes of venomous species to understand the mechanisms and processes that drive their toxin diversity and venom evolution.

Group expertise / Methods

  • Genomics, transcriptomics, proteomics (In-house pipeline)
  • Expression of animal toxins bioassays, activity tests
  • Evolutionary venomics, evolution, phylogeny, phylogenomics
  • Expression of animal toxins: recombinant production and cell free expression of animal toxins
  • Several bioassays (see progress)
  • HPLC and UHPLC based Mass Spectrometry (cooperation with Christoph Hartwig, Fraunhofer IME, Giessen)
  • LC-MALDI coupling and localization of disulfide linkages (cooperation with the group of Günter Lochnit, Institute for Biochemistry, Giessen).