Influence of anthropogenic substances on germ cell mutation rate

Influence of anthropogenic substances on the germ cell mutation rate   

Markus Pfenninger


Tests of mutagenicity are currently performed with single celled eukaryotes or prokaryotes (e.g. ISO 13829 (2000); ISO 16420 (2005) or conclude from phenotypic effects on mutagenicity of substances. Since the mutation and repair mechanisms of multicellular eukaryotes (including our species) differs substantially, the transfer of the results from these test is questionable and remains untested. The development of a new method now allows the inference of mutation rates with reasonable effort. Genom-wide screening for base substitutions, deletions, insertions and chromosomal aberrations promises a far higher sensitivity than current mutagenicity tests. It is our goal to establish a new paradigm in the ecotoxicological assessment of anthropogenic substances in international frameworks (e.g. ISO). To achieve this, we will work with the genomes of ecotoxicological model species like Chironomus riparius, Folsomia candida, Eisenia andrei and Daphnia galeata. .


De novo genomes 2
Re- sequenced genomes several hundred (to 20X)
Total genomes: many
Total data: several dozen Tera bases


SPECIES: Date (due) NCBI Coverage Quality (planned)  
Harlequin fly (Chironomus riparius) 01.01.2018 Yes >100x  
Waterflea (Daphnia galeata) 01.08.2018 No (~130x)  
Earthworm (Eisenia andrei) 01.01.2019 No (~100x)